Computational design of antagonist peptides based on the structure of secreted frizzled-related protein-1 (SFRP1) aiming to inhibit Wnt signaling pathway

Secreted frizzled-related proteins (SFRP) are glycoproteins containing a so-called frizzled-like cysteine-rich area. This area permits them to bind to Wnt ligands or frizzled (FzD) receptors, making potent regulators of Wnt signaling. As Wnt signaling is usually altered in most cancers, it isn’t shocking that Wnt regulators akin to SFRP proteins are sometimes differentially expressed in the tumor microenvironment, each in a metastatic and non-metastatic setting. Indeed, SFRP2 is proven to be particularly upregulated in the tumor vasculature of a number of varieties of most cancers. Several research investigated the purposeful position of SFRP2 in the tumor vasculature, displaying that SFRP2 binds to FzD receptors on the floor of tumor endothelial cells. This prompts downstream Wnt signaling and which is, thereby, stimulating angiogenesis. Interestingly, not the well-known canonical Wnt signaling pathway, however the noncanonical Wnt/Ca²⁺ pathway appears to be a key participant on this occasion.

In tumor fashions, the pro-angiogenic impact of SFRP2 could possibly be counteracted by antibodies concentrating on SFRP2, with out the incidence of toxicity. Since tumor angiogenesis is a vital course of in tumorigenesis and metastasis formation, particular tumor endothelial markers akin to SFRP2 present nice promise as targets for anti-cancer therapies. This overview discusses the position of SFRP2 in noncanonical Wnt signaling and tumor angiogenesis, and highlights its potential as anti-angiogenic therapeutic goal in most cancers. Haemophilus parasuis induces extreme acute systemic an infection in pigs, characterised by fibrinous polyserositis, polyarthritis and meningitis. Our earlier examine demonstrated that H. parasuis induced the activation of p38 mitogen-activated protein kinase (MAPK) pathway, rising the expression of proinflammatory genes and mediating H. parasuis-induced irritation. Moreover, Wnt/β-catenin signaling activation induced by H. parasuis disrupts the adherens junction between epithelial cells and initiates the epithelial-mesenchymal transition (EMT).

In the current examine, p38 MAPK was discovered to be concerned in the accumulation of nuclear location of β-catenin throughout H. parasuis an infection in PK-15 and NPTr cells, by way of modulating the expression of dickkofp-1 (DKK-1), a damaging regulator of Wnt/β-catenin signaling. We generated DKK-1 knockout cell strains by CRISPR/Cas9-mediated genome enhancing in PK-15 and NPTr cells, and located that knockout of DKK-1 led to the dysfunction of p38 MAPK in regulating Wnt/β-catenin signaling exercise in H. parasuis-infected cells. Furthermore, p38 MAPK exercise was impartial of the activation of Wnt/β-catenin signaling throughout H. parasuis an infection. This is the first examine to discover the crosstalk between p38 MAPK and Wnt/β-catenin signaling throughout H. parasuis an infection. It supplies a extra complete view of intracellular signaling pathways throughout pathogenic bacteria-induced acute irritation.

Wnt signaling dysregulation promotes tumorigenesis in colorectal most cancers (CRC). We investigated the position of PTPRF, a receptor-type tyrosine phosphatase, in regulating Wnt signaling in CRC. Knockdown of PTPRF decreased cell proliferation in patient-derived main colon most cancers cells and established CRC cell strains. In addition, the charge of proliferation in addition to colony formation skill had been considerably decreased in tumor organoids grown in 3D, whereas the quantity of differentiated tumor organoids had been markedly elevated. Consistently, knockdown of PTPRF resulted in a lower in the expression of genes related to most cancers stem cells downstream of Wnt/β-catenin signaling.

Active AHCY, human recombinant
7527-50	Biovision		EUR 479

Active Recombinant Human CETP
7606-100	Biovision		EUR 805

Active Recombinant Human CETP
7606-1000	Biovision		EUR 4345

Active Recombinant Human CETP
7606-20	Biovision		EUR 262

Active SIRT2, human recombinant
7698-10	Biovision		EUR 262

Active SIRT2, human recombinant
7698-1000	Biovision		EUR 4394

Active SIRT2, human recombinant
7698-50	Biovision		EUR 457

Active SIRT7, human recombinant
7846-10	Biovision		EUR 218

Active SIRT7, human recombinant
7846-50	Biovision		EUR 588

Irisin, Active, human recombinant
7852-10	Biovision		EUR 370

Irisin, Active, human recombinant
7852-50	Biovision		EUR 1077

HER2, Active, Human Recombinant
8011-5	Biovision		EUR 240

HER2, Active, Human Recombinant
8011-50	Biovision		EUR 1132

PRMT1, human recombinant (Active)
4865-10	Biovision		EUR 153

PRMT1, human recombinant (Active)
4865-1000	Biovision		EUR 3834

PRMT1, human recombinant (Active)
4865-50	Biovision		EUR 392

GAPDH, Active, human recombinant
P1100-10	Biovision		EUR 142

GAPDH, Active, human recombinant
P1100-50	Biovision		EUR 392

Active Cathepsin S, human recombinant
7526-10	Biovision		EUR 338

Active Cathepsin S, human recombinant
7526-50	Biovision		EUR 1126

Active Cathepsin S, human recombinant
7526-500	Biovision		EUR 9130

Active Cathepsin B, human recombinant
7580-1000	Biovision		EUR 9783

Active Cathepsin B, human recombinant
7580-5	Biovision		EUR 294

Active Cathepsin B, human recombinant
7580-50	Biovision		EUR 1132

Cathepsin K, Active, human recombinant
7600-5	Biovision		EUR 392

Cathepsin K, Active, human recombinant
7600-50	Biovision		EUR 2279

MMP-9, Active, human recombinant
7867-1000	Biovision		EUR 501

MMP-9, Active, human recombinant
7867-500	Biovision		EUR 332

Active BCL2L1 Protein, human recombinant
7885-10	Biovision		EUR 256

Active BCL2L1 Protein, human recombinant
7885-50	Biovision		EUR 691

MMP-14, Active, Human Recombinant
8009-10	Biovision		EUR 316

MMP-14, Active, Human Recombinant
8009-50	Biovision		EUR 805

Active Cathepsin D, Human Recombinant
9229-10	Biovision		EUR 245

Active Cathepsin D, Human Recombinant
9229-100	Biovision		EUR 1132

Active Cathepsin D, Human Recombinant
9229-50	Biovision		EUR 735

MMP-9, Active, Human Recombinant
P1567-10	Biovision	10 µg	EUR 348

MMP-9, Active, Human Recombinant
P1567-50	Biovision	50 µg	EUR 510

5-Lipoxygenase, Active, Human Recombinant
P1373-200	Biovision		EUR 316

Recombinant Human Activin-A Active
7-00004	CHI Scientific	1ug	Ask for price

Recombinant Human Activin-A Active
7-00005	CHI Scientific	5ug	Ask for price

Recombinant Human Activin-A Active
7-00006	CHI Scientific	100µg	Ask for price

Recombinant Human Activin-B Active
7-00010	CHI Scientific	1ug	Ask for price

Recombinant Human Activin-B Active
7-00011	CHI Scientific	5ug	Ask for price

Recombinant Human Activin-B Active
7-00012	CHI Scientific	100µg	Ask for price

Human CellExp? HDAC6, Human Recombinant, Active
P1265-50	Biovision		EUR 446

Human CellExp? HDAC8, Human Recombinant, Active
P1266-50	Biovision		EUR 446

Human CellExp? HDAC11, Human Recombinant, Active
P1267-50	Biovision		EUR 446

PRMT1, mouse recombinant (Active)
4868-10	Biovision		EUR 153

PRMT1, mouse recombinant (Active)
4868-1000	Biovision		EUR 3834

PRMT1, mouse recombinant (Active)
4868-50	Biovision		EUR 392

Active SIRT6 (GST-tagged), human recombinant
7697-100	Biovision		EUR 479

Active SIRT6 (GST-tagged), human recombinant
7697-1000	Biovision		EUR 3013

Active SIRT6 (GST-tagged), human recombinant
7697-20	Biovision		EUR 272

Active SIRT6 (His-tagged), human recombinant
7699-100	Biovision		EUR 425

Active SIRT6 (His-tagged), human recombinant
7699-1000	Biovision		EUR 2545

Active SIRT6 (His-tagged), human recombinant
7699-20	Biovision		EUR 218

Active Sonic Hedgehog (SHH), human recombinant
4010-100	Biovision		EUR 936

Active Sonic Hedgehog (SHH), human recombinant
4010-1000	Biovision		EUR 4432

Active Sonic Hedgehog (SHH), human recombinant
4010-25	Biovision		EUR 327

Activin-A Human Recombinant Protein, Active
PROTP08476-2	BosterBio	Regular: 10ug	EUR 317
Description: Active form Activin-A Human Recombinant produced in e.coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 117 amino acids and having a molecular weight of 26.2kDa.;The Active form Activin-A is purified by standard chromatographic techniques.

Activin-B Human Recombinant Protein, Active
PROTP09529	BosterBio	Regular: 10ug	EUR 317
Description: Activin B human Recombinant produced in Nicotiana benthamiana plant is a beta-B single chain (aa 293-406) containing 123 amino acids (molecular formula C615H910N178O177S12). Activin B is fused to a 10-His-tag at the N-terminal having the total molecular mass of 14kDa and purified by standard chromatographic techniques.

Recombinant Human Active Focal Adhesion Kinase
7-03955	CHI Scientific	1µg	Ask for price

Recombinant Human Active Focal Adhesion Kinase
7-03956	CHI Scientific	5µg	Ask for price

Recombinant Human Active Focal Adhesion Kinase
7-03957	CHI Scientific	10µg	Ask for price

Recombinant Human Alpha-1 Antitrypsin, Active
7-06361	CHI Scientific	20µg	Ask for price

Recombinant Human Alpha-1 Antitrypsin, Active
7-06362	CHI Scientific	100µg	Ask for price

Recombinant Human Alpha-1 Antitrypsin, Active
7-06363	CHI Scientific	1mg	Ask for price

Active Human Caspases Group I, Active Human
K241-3-25	Biovision		EUR 370

Human ACTIVE GLP1(ACTIVE GLP1) ELISA Kit
QY-E05350	Qayee Biotechnology	96T	EUR 361

Oxalate Decarboxylase, Active Bacterial Recombinant
7262-100	Biovision		EUR 446

Oxalate Decarboxylase, Active Bacterial Recombinant
7262-1000	Biovision		EUR 2931

Oxalate Decarboxylase, Active Bacterial Recombinant
7262-20	Biovision		EUR 245

Active Caspase-1, rat recombinant
7524-100	Biovision		EUR 555

Active Caspase-1, rat recombinant
7524-25	Biovision		EUR 338

Cathepsin K, Active, mouse recombinant
7597-5	Biovision		EUR 359

Cathepsin K, Active, mouse recombinant
7597-50	Biovision		EUR 2170

Cathepsin K, Active, rat recombinant
7598-5	Biovision		EUR 359

Cathepsin K, Active, rat recombinant
7598-50	Biovision		EUR 2170

Aprotinin, Active, Bovine Recombinant (AOF)
7866-1	Biovision		EUR 115

Aprotinin, Active, Bovine Recombinant (AOF)
7866-5	Biovision		EUR 158

Active HRV14 3C Protease Recombinant
8012-100	Biovision		EUR 588

Active HRV14 3C Protease Recombinant
8012-20	Biovision		EUR 207

PDK, Active, M. rosea Recombinant
P1161-50	Biovision		EUR 398

D-Serine Dehydratase, Active, Recombinant
P1193-200	Biovision		EUR 457

D-Serine Dehydratase, Active, Recombinant
P1193-600	Biovision		EUR 914

Homocysteine ?,?-Lyase (rHCYase), Active, Recombinant
P1117-200	Biovision		EUR 425

Recombinant WNV Active E Antigen
VAng-Lsx0573-inquire	Creative Biolabs	inquire	Ask for price
Description: WNV Active E Antigen, recombinant protein from E. coli.

Recombinant MeV Active Nucleocapsid Protein
VAng-Lsx0400-inquire	Creative Biolabs	inquire	Ask for price
Description: Measles Active Nucleocapsid, recombinant protein from E. coli.

Active Transglutaminase 2 (His-tagged), human recombinant
7700-10	Biovision		EUR 233

Active Transglutaminase 2 (His-tagged), human recombinant
7700-100	Biovision		EUR 592

Fructose 1,6-bisphophatase 1, Active, Human Recombinant
P1269-10	Biovision		EUR 153

Fructose 1,6-bisphophatase 1, Active, Human Recombinant
P1269-50	Biovision		EUR 479

Thioredoxin isoform 1 (Trx), Active, Human Recombinant
P1039-100	Biovision		EUR 240

Activin-A Human Recombinant Protein, Plant-Active
PROTP08476-3	BosterBio	Regular: 5ug	EUR 317
Description: Active form Activin-A Human Recombinant produced in Plant is a homodimeric, glycosylated, polypeptide chain containing 2 x 116 amino acids and having a molecular weight of 27.4kDa.;The Active form Activin-A is fused to a 6-His tag at N-terminus and purified by standard chromatographic techniques.

Recombinant Human Casein Kinase 2 beta Active
7-03892	CHI Scientific	3µg	Ask for price

Recombinant Human Casein Kinase 2 beta Active
7-03893	CHI Scientific	10µg	Ask for price

Recombinant Human Casein Kinase 2 beta Active
7-03894	CHI Scientific	20µg	Ask for price

Purified Recombinant Human BMP3 protein, Biologically active
BMP35-R-10	Alpha Diagnostics	10 ug	EUR 408

Purified Recombinant Human BMP4 protein, Biologically active,
BMP45-R-10	Alpha Diagnostics	10 ug	EUR 895

Purified Recombinant Human BMP4 protein, Biologically active
BMP45-R-5	Alpha Diagnostics	5 ug	EUR 529

Purified Recombinant Human BMP6 protein, Biologically active
BMP65-R-20	Alpha Diagnostics	20 ug	EUR 895

Recombinant purified, human NFkB-p65 protein, active
NFKB651-R-5	Alpha Diagnostics	5 ug	EUR 651

Recombinant (E.Coli) Human ERK1/MAPK3 Active Enzyme
RP-670	Alpha Diagnostics	3 ug	EUR 590

Recombinant (E.Coli) Human ERK2/MAPK1 Active Enzyme
RP-672	Alpha Diagnostics	1 ug	EUR 590

Purified, recombinant Human TACE control protein (active)
TACE15-R-10	Alpha Diagnostics	10 ug	EUR 712

Human Recombinant VEGF121 Protein (Sf9), biologically active
VEGF24-R-10	Alpha Diagnostics	10 ug	EUR 445

Human Recombinant VEGF121 Protein (Sf9), biologically active
VEGF24-R-100	Alpha Diagnostics	100 ug	EUR 2425

Human Recombinant VEGF121 Protein (E.Coli), biologically active
VEGF25-R-10	Alpha Diagnostics	10 ug	EUR 445

Human Recombinant VEGF121 Protein (E.Coli), biologically active
VEGF25-R-100	Alpha Diagnostics	100 ug	EUR 2425

Human Recombinant VEGF165 Protein (E.Coli), biologically active
VEGF26-R-10	Alpha Diagnostics	10 ug	EUR 445

Human Recombinant VEGF165 Protein (E.Coli), biologically active
VEGF26-R-100	Alpha Diagnostics	100 ug	EUR 2242

Human Recombinant VEGF165 Protein (E.Coli), biologically active
VEGF26-R-1000	Alpha Diagnostics	1000 ug	EUR 9185

Human Recombinant VEGF165 Protein (Sf9), biologically active
VEGF27-R-10	Alpha Diagnostics	10 ug	EUR 445

Human Recombinant VEGF165 Protein (HEK), biologically active
VEGF27-R-100	Alpha Diagnostics	100 ug	EUR 2425

Human Recombinant VEGF165 Protein (Sf9), biologically active
VEGF27-R-1000	Alpha Diagnostics	1000 ug	EUR 9185

Human CellExp? HDAC5, Catalytic Domain, Human Recombinant, Active
P1268-50	Biovision		EUR 446

CSTA Human, Cystatin-A Human Recombinant Protein, Active
PROTP01040	BosterBio	Regular: 20ug	EUR 317
Description: Cystatin A Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 118 amino acids (1-98a.a.) and having a molecular mass of 13.1 kDa.;The Cystatin A is fused to a 20 amino acid His tag at N-terminus and purified by proprietary chromatographic techniques.

Human, Activin-A Human Recombinant Protein, HEK-Active
PROTP08476-1	BosterBio	Regular: 10ug	EUR 317
Description: Activin-A Human Recombinant produced in HEK cells is a non-glycosylated disulfide-linked homodimer, having a total molecular weight of 25kDa.;The Activin-A is purified by proprietary chromatographic techniques.

PGP Human, Phosphoglycolate Phosphatase Human Recombinant Protein, Active
PROTA6NDG6-1	BosterBio	Regular: 10ug	EUR 317
Description: PGP Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 345 amino acids (1-321a.a) and having a molecular mass of 36.5kDa.;PGP is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

GDA Human, Guanine Deaminase Human Recombinant Protein, Active
PROTQ9Y2T3-2	BosterBio	Regular: 10ug	EUR 317
Description: GDA Human Recombinant produced in E. coli is a single polypeptide chain containing 477 amino acids (1-454) and having a molecular mass of 53kDa.;GDA is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

GLUL Human, Glutamine Synthetase Human Recombinant Protein, Active
PROTP15104-1	BosterBio	Regular: 10ug	EUR 317
Description: GLUL Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 373 amino acids (1-373) and having a molecular mass of 42kDa.

GLO1 Human, Glyoxalase-I Human Recombinant Protein, Active
PROTQ04760-1	BosterBio	Regular: 10ug	EUR 317
Description: Glyoxalase-I Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 184 amino acids and having a molecular mass of 20.7 kDa. ;Glyoxalase-1 is purified by proprietary chromatographic techniques.

CST3 Human, Cystatin-C Human Recombinant Protein, Active
PROTQ6FGW9-1	BosterBio	Regular: 10ug	EUR 317
Description: Cystatin-C Human Recombinant produced in HEK cells is a non-glycosylated monomer, having a molecular weight of approximately 13kDa.;The Cystatin-C is purified by proprietary chromatographic techniques.

MGLL Human, Monoglyceride Lipase Human Recombinant Protein, Active
PROTQ99685-1	BosterBio	Regular: 5ug	EUR 317
Description: MGLL Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 333 amino acids (1-313 a.a.) and having a molecular mass of 36.4kDa. The MGLL is purified by proprietary chromatographic techniques.

Human Tau proteins ELISA kit
CSB-E12011h-24T	Cusabio	1 plate of 24 wells	EUR 165
Description: Quantitativesandwich ELISA kit for measuring Human Tau proteins in samples from serum, plasma, cerebrospinalfluid (CSF). A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Tau proteins ELISA kit
1-CSB-E12011h	Cusabio	EUR 900.00 EUR 5476.00 EUR 2900.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Tau proteins in samples from serum, plasma, cerebrospinalfluid(CSF). Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Lys proteins Antibody
ABD2705	Lifescience Market	100 ug	EUR 438

Lys proteins Antibody
DF2705	Affbiotech	200ul	EUR 304
Description: Lys proteins antibody detects endogenous levels of total Lys proteins.

Bone Matrix Proteins
5-00844	CHI Scientific	4 x 5mg	Ask for price

Bone Matrix Proteins
20-abx265965	Abbexa	EUR 384.00 EUR 592.00 EUR 300.00	10 mg 25 mg 5 mg

Lys proteins Antibody
abx219526-100ug	Abbexa	100 ug	EUR 439

Active Recombinant Human beta-Secretase 1 - BACE-1
7609-1000	Biovision		EUR 9783

Active Recombinant Human beta-Secretase 1 - BACE-1
7609-5	Biovision		EUR 262

Active Recombinant Human beta-Secretase 1 - BACE-1
7609-50	Biovision		EUR 697

Recombinant Human MAPK1, Active Protein, Untagged, E.coli-1ug
QP14023-1ug	EnQuireBio	1ug	EUR 155

Recombinant Human MAPK1, Active Protein, Untagged, E.coli-5ug
QP14023-5ug	EnQuireBio	5ug	EUR 201

Recombinant Human MAPK1, Active Protein, Untagged, E.coli-100ug
QP14023-EC-100ug	EnQuireBio	100ug	EUR 2059

Recombinant Human Mitogen-Activated Protein Kinase 3, Active
7-03937	CHI Scientific	3µg	Ask for price

Treating PTPRF knockdown cells with GSK3 inhibitor rescued the expression of Wnt goal genes suggesting that PTPRF capabilities upstream of the β-catenin destruction complicated. PTPRF was discovered to work together with LRP6 and silencing PTPRF largely decreased the activation of LRP6. Interestingly, this PTPRF-mediated activation of Wnt signaling was blocked in cells handled with clathrin endocytosis inhibitor. Furthermore, knockdown of PTPRF inhibited xenograft tumor development in vivo and decreased the expression of Wnt goal genes. Taken collectively, our research establish a novel position of PTPRF as an oncogenic protein phosphatase in supporting the activation of Wnt signaling in CRC.