- The use of a comb with wider wells to pour agarose gel
- digesting about 1-2ug DNA (7-14 uL of Miniprep a)
- Run the gel at 80V for as long as possible (it will probably take more than one hour to run)
- while the gel is running, adjust the heating block to 50 degrees; get eppendorf tube, spatula, razor ready; weigh empty eppendorf tube and recorded its weight
- after the gel is finished running, the gel into the apparatus for cutting gel
- UV light up to preparative (Use Dont analytically, as a high-energy UV will damage DNA)
- excise out the minimum number of agarose and place it into the tube eppendorf
- weigh samples in eppendorf tube and subtract out the weight of the tube eppendorf
- follow the instructions in the manual extraction of gel of any company that you buy from (eg Qiagen)
