Posted By: Judith Reynolds
October 14, 2020
- Wash cells with cold PBS.
- resuspend the cells in ice. cold TE buffer (10mm Tris, pH 7.4, 2mm EDTA)
- Wash 2x in ice-cold TE (rotating in 40,000 x g, 8 minutes each time)
- For 36 mM CHAPS in PBS with stirring in the cold (for example on ice or in a cold room), adding the cells resuspended in ~ 1 mL TE buffer dropwise.
- dissolve the protein with stirring for 30 minutes in a cold room after adding cells was added dropwise.
- Spin the sample is dissolved in the 100,000 x g, 10 min.
- Use the supernatant, which contains dissolved proteins, for further analysis or purification.
