Protocol on How to Solubilize Membrane Proteins with CHAPS
Wash cells with cold PBS.resuspend the cells in ice. cold TE buffer (10mm Tris, pH 7.4, 2mm EDTA)Wash 2x in ice-cold TE (rotating in 40,000 x g, 8 minutes each time)For 36 mM CHAPS in…
Wash cells with cold PBS.resuspend the cells in ice. cold TE buffer (10mm Tris, pH 7.4, 2mm EDTA)Wash 2x in ice-cold TE (rotating in 40,000 x g, 8 minutes each time)For 36 mM CHAPS in…